14879
Sigma
BIS-TRIS
BioUltra, ≥99.0% (NT)
| Synonym: | 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane |
| CAS Number: | 6976-37-0 |
| Linear Formula: | C8H19NO5 |
| Molecular Weight: | 209.24 |
| Beilstein Registry Number: | 2205275 |
| EC Number: | 230-237-7 |
| MDL number: | MFCD00002853 |
| PubChem Substance ID: | 24848919 |
Description
| Other Notes | Effective buffer for the assay of creatine kinase1; Effectively protects haemoglobin during freeze-drying2; Catholyte in IEF of 2-D gel electrophoresis3. |
| Easily compare specifications for BIS-TRIS products with the BIS-TRIS specification table. |
Properties
| product line | BioUltra |
| assay | ≥99.0% (NT) |
| total impurities | insoluble matter, passes filter test |
| ign. residue | ≤0.3% |
| loss | ≤0.5% loss on drying, 20 °C (HV) |
| pH | 9.5-11.0 (25 °C, 1 M in H2O) |
| useful pH range | 5.8 - 7.2 |
| pKa (25 °C) | 6.5 |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| abs. | absorption |
| λ | 1 M in H2O |
| UV absorption | λ: 280 nm Amax: 0.15 |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
